补中益气汤含药血清对Lewis肺癌细胞增殖和凋亡的影响

Effect of Serum Medicated of Buzhongyiqi Decoction on Proliferation and Apoptosis of Lung Cancer Cell

目的:观察补中益气汤含药血清对Lewis肺癌细胞增殖的抑制作用及其对细胞凋亡的影响,探讨补中益气汤治疗肿瘤的机制。方法:以补中益气汤给大鼠灌胃,腹主动脉采血并制备血清。MTT法检测含药血清对肺癌细胞增殖的抑制作用,流式细胞仪测定肺癌细胞细胞凋亡。结果:补中益气汤不同浓度含药血清组与空白对照组比较均可明显抑制肺癌细胞体外增殖,差异有统计学意义(P<0.05);补中益气汤不同浓度含药血清组Lewis肺癌细胞凋亡率与空白对照组比较,明显升高,且随着含药血清浓度的增加,细胞凋亡率明显上升,具有浓度依赖性。结论:补中益气汤含药血清对肺癌细胞体外增殖有明显的抑制作用,且可促进肿瘤细胞凋亡。

Objective ： Observe the proliferation inhibition of serum medicated of Buzhongyiqi decoction on lung cancer cell and its influ- ence on cell apoptosis and explore the mechanism of Buzhongyiqi decoction in treating tumor. Methods ： Rats were given intragastric ad- ministration of Buzhongyiqi decoction, abdominal aortic blood serum and preparation. Use MTT method to detect the proliferation inhibi- tion of drug-containing serum for lung cancer cell and flow cytometry instrument to measure lung cancer cell apoptosis. Results ： Com- pared with blank control group, different concentrations of serum medicated of Buzhongyiqi decoction can obviously inhibit lung cancer cells in vitro proliferation ,the difference was statistically significant（ P 〈 0.05 ）. Compared with blank control group, the cell apoptosis rate of different concentrations of serum medicated of Buzhongyiqi decoction increased significantly. As the increase of medicated serum concentration, cell apoptosis rate increased significantly with the concentration dependence. Conclusion ： Serum medicated of Buzhongyiqi decoction has obvious inhibitory effect on lung cancer cells in vitro proliferation and can promote apoptosis of tumor cells.