云连总生物碱提取工艺优化研究

Study on the Mization of Extraction Technology of Total Alkaloids from Coptis Teeta Wall

目的:筛选云连总生物碱最优提取工艺。方法:利用正交试验筛选最优提取工艺,通过大孔吸附树脂进行纯化。结果:通过对正交试验结果进行分析,得到最佳提取工艺:8倍量体积分数80%乙醇提取3次,每次1 h,经纯化,云连提取物浸膏中总生物碱含量达到55%以上。结论:云连总生物碱最佳提取工艺稳定可行。

Objective ： To optimize the extraction process of total alkaloids from coptis teeta wall. Methods ： The optimum extraction process was done by orthogonal experiment and purified by macroporous resin. Results：The optimum extraction process was done by analyzing the results of orthogonai experiments, using 8 times the amount of g0 % alcohol extract for 3 times ,60 rain every time ,the total alkaloids content found in extractum coptis teeta wall reached more than 55 %. Conclusion：The mization of extraction technology of total alkaloids from Coptis Teeta Wall is feasible.