大孔吸附树脂分离纯化地骨皮总黄酮实验研究

Separation and Purification of Total Flavonoids from Lycii Cortex by Column Chromatography of Macroreticular Resin

目的:研究并优化大孔吸附树脂分离纯化地骨皮总黄酮的方法。方法:先以总黄酮含量为考察指标,从3种不同型号的树脂中筛选出分离纯化地骨皮总黄酮的最佳树脂,再对最佳树脂吸附工艺参数进行全面优化。结果:AB-8型大孔吸附树脂对地骨皮总黄酮的吸附与解析性能最好,确定最佳洗脱条件为体积分数50%乙醇洗脱,溶剂用量2 BV(树脂床体积)。结论:AB-8型大孔吸附树脂可有效地分离纯化地骨皮总黄酮。

Objective：To optimize the separation and purification of total flavonoids from Lycii Cortex by macroporous resin. Methods： Three types of macroporous resins were used to separate and purify flavonoids. Contents of the total flavonoids were compared in order to find the optimum macroporous resin, and its technical process was comprehensive optimized. Results：AB-8 was selected for its excellent adsorption and desorption properties. The best elution solution technical process was 2BV of 50% ethanol. Conlusion： The total fla- vonoids of Lycii Cortex can be effectively separated and purificated by AB-8 macroporous resin.