脑心综合征大鼠血清B-FABP H-FABP的变化及意义

Changes of B-FABP and H-FABP levels in rat serum with cerebral-cardiac syndrome

目的探讨急性脑梗死并发脑心综合征大鼠血清脑型脂肪酸结合蛋白(B-FABP)、心型脂肪酸结合蛋白(HFABP)水平的动态变化及意义。方法采用线栓法栓塞大鼠右侧大脑中动脉,连续监测大鼠大脑中动脉栓塞后2h标准Ⅱ导联心电图,将实验大鼠分为脑心综合征组、单纯脑梗死组、假手术对照组,检测血清中B-FABP、H-FABP水平。结果脑心综合征组、单纯脑梗死组两者分别与假手术对照组相比较,血清B-FABP水平升高,P<0.05;血清H-FABP水平差异无统计学意义(P>0.05)。脑心综合征组与单纯脑梗死组比较,血清B-FABP、H-FABP水平差异均无统计学意义(P>0.05)。Pearson直线相关分析结果显示,血清H-FABP水平与B-FABP水平呈正相关(r=0.717,P<0.001)。结论 B-FABP对脑梗死的早期诊断有一定意义,B-FABP、H-FABP联合心电图对脑心综合征的诊断有一定作用。

Objective To observe the changes of B‐FABP and H‐FABP levels in rat serum with cerebral‐cardiac syndrome following acute cerebral infarction.Methods Right middle cerebral artery of rat was occluded （MCAO） and Ⅱ lead ECG was monitored continuously within 2 h ,the cerebral‐cardiac syndrome group ,the only cerebral infarction group and the sham opera‐tion group. Extracting angular venous blood and detect the serum of rats in B‐FABP and H‐FABP levels. Results The B‐FABP levels in cerebral‐cardiac syndrome group and the only cerebral infarction group were all significantly higher than those of the sham operation group ,P〈0.05 ;but the level of serum H‐FABP had no statistics difference between every group ,P〉0.05. The B‐FABP and H‐FABP levels in cerebral‐cardiac syndrome group had no statistics difference than the only cerebral infarc‐tion group ,P〉0.05. The serum B‐FABP concentrations had a positive correlation with H‐FABP concentrations（r=0.717 ,P〈0 .001）.Conclusion H‐FABP can be used as a noninvasive marker for ACI. Unite H‐FABP and B‐FABP has certain signifi‐cance to the diagnosis of cerebral‐cardiac syndrome.