疏肝补肾法对大鼠乳腺癌癌前病变模型EGFR/STAR3细胞通路影响

Effects of EGFR/STAR3 Cell Pathway via Shugan Bushen on Rats Model with Precancerous Lesions of Breast Cancer

目的:研究疏肝补肾中药对大鼠乳腺癌癌前病变模型EGFR/STAR3细胞通路的影响,探讨疏肝补肾中药对于阻断大鼠乳腺癌癌前病变的可能机制。方法:SD大鼠二甲基苯恩法造模后分为模型组、消核冲剂低剂量组、消核冲剂高剂量组,每组20只分别中药处理,光镜观察、免疫组化方法检测各组的大鼠模型乳腺组织病理形态、ER、PR表达及EGF、EGFR、STAT3蛋白表达。结果:光镜观察结果显示中药可部分逆转及阻断乳腺癌癌前病变进程;免疫组化方法显示中药组较模型组EGFR、STAR3表达降低(P<0.05),作用随剂量增加而增强。结论:疏肝补肾中药可以通过阻断EGFR/STAR3细胞信号传导通路的途径抑制雌激素分泌从而达到逆转阻断乳腺非典型增生发展的作用,使其恢复正常状态,并且作用程度随着剂量的增加而增加。

Objective ： To study the shu gan bu shen in traditional Chinese medicine for breast precancerous lesions in rats model of el＇feet of EGFR/STAR3 cellular pathways, explore the Shugan Bushen in traditional Chinese medicine for uncle blocking the possible mechanism of breast precancerous lesions. Methods： 80 SD rats, blank control group of 20,60 only after the building is divided into model group, nuclear infusion of nuclear infusion of low dose group, high dose group, each group 20 processing, respectively to detect different building methods the rat model of breast tissue ER, PR expression and the expression of EGF and EGFR and STAT3 protein by immunohistoehemical and western blot method. Results： the immunohistochemieal method and western blot method, in situ hybridization method all showed Chinese medicine group compared with model group of ER, PR, EGFR, STAR3 expression reduced （ P〈0.05 ） .Conclusion ： the Shugan Bushen can Chinese traditional medicine by blocking EGFR/ STAR3 signaling pathways way estrogen secretion to reverse by blocking the effect of atypical hyperplasia of mammary gland development, make it back to normal state, and the degree of effect increases with the increase of the dose.