冠心颗粒的制备与质量分析

Preparation and quality analysis of Guanxin particles

采用乙醇回流法提取丹参、赤芍等药材，加入辅料（蔗糖∶糊精＝2∶1）制备冠心颗粒，采用RP-HPLC法测定颗粒的有效成分芍药苷、丹参素、丹酚酸B和丹酚酸A，以Agilent TC-C18（150 mm ×4．6 mm，5μm）为分析柱，以甲醇-0．2％磷酸溶液为流动相梯度洗脱，流速1．0 mL／min，柱温28℃。测得芍药苷、丹参素、丹酚酸B、丹酚酸A分别在5．44～87．04，6．68～106．88，9．48～151．68，3．06～48．96μg／mL内线性良好，r≥0．9992。

Guanxin particles were prepared by adding different proportions sucrose and dextrin （2∶1） into the extractives from ethanol backflow extraction .The effective components such as paeoniflorin ,Danshen-su,salvianolic acid B and A were determined using RP-HPLC.The Agilent TC-C18 （150 mm ×4.6 mm, 5 μm） column was used,with methanol-0.2%phosphoric acid solution as mobile phase and gradient elu-tion.The flow rate was 1.0 mL/min at 28 ℃.The linear relationships were good in the range of 5.44～87.04 μg/mL for paeoniflorin,6.68～106.88 μg/mL for Danshensu,9.48～151.68 μg/mL for salvian-olic acid B and 3.06～48.96μg/mL for salvianolic acid A,respectively.The correlation coefficients were no less than 0.999 2.