含量测定和指纹图谱结合LC-MS技术整体评价银杏叶片的质量

Quantitative and qualitative evaluation on tablets of Ginkgo biloba leaves using fingerprint and LC-MS analysis

建立银杏叶片合理质量控制的方法，评价市售不同厂家银杏叶片的质量。收集市售银杏叶片，采用HPLC对其进行总黄酮醇苷和萜类内酯含量测定，根据2010年版《中国药典》一部方法进行测定，2批银杏叶片总黄酮醇苷含量不合格，3批银杏叶片萜类内酯含量不合格；建立HPLC指纹图谱检测方法对其进行检测，不同厂家生产的16批银杏叶片HPLC指纹图谱标记出31个共有峰，与生成的对照指纹图谱相似度为0．763～0．989；采用LC-MS对指纹图谱色谱峰进行指认，鉴定出31个黄酮类成分。以总黄酮醇苷含量、萜类内酯含量和指纹图谱结合LC-MS技术整体评价银杏叶片的质量，为银杏叶片质量研究提供一些研究思路。

A reasonable method for the quality control of tablets of Ginkgo biloba leaves was established in this paper. The total fla- vonol glycosides and terpene lactones of G. biloba tablets were quantified by HPLC. Totally, 16 batches of the commercially available tablets of G. biloba leaves were determined. Among of them, 2 batches were unqualified in the content of total flavonol glycosides, and 3 batches were unqualified in the content of terpene lactones. A validated HPLC fingerprint method was established to evaluate the com- mercially available tablets of G. biloba leaves with the assistance of LC-MS. Sixteen batches showed the similarity of 0. 763-0. 989. There were 31 fingerprint chromatogram peaks were identified as flavonoids compositions by LC-MS. This provides a research idea for the quality control of tablets of G. biloba leaves.