短柄小连翘中乙酸乙酯部位化学成分的研究

Ethyl acetate-soluble chemical constituents from branch of Hypericum petiolulatum

采用乙醇提取和液液萃取的方法对短柄小连翘茎枝进行了提取。通过硅胶柱色谱、Sephadex LH-20柱色谱、反相中压柱色谱和高效液相等多种色谱方法相结合，从短柄小连翘乙醇提取物的乙酸乙酯部位中分离得到了7个双环氧木脂素化合物。经MS和NMR等波谱技术分别鉴定为松脂醇（pinoresinol，1），5’-甲氧基-松脂素（medioresinol，2），8-乙酰基松脂醇（8-ace-toxypinoresinol，3），表松脂醇（epipinoresinol，4），（＋）-丁香树脂醇[（＋）-syringaresinol（5）]、（＋）-1-羟基丁香树脂醇[（＋）-1-hydmxysyringaresin01（6）]和erythro-buddlenolE（7）。化合物1～7均为首次从该植物中分离得到。抗氧化活性筛选结果显示，化合物7在1×10^-6mol·L^-1浓度下，对Fe^2＋-Cys诱导大鼠肝微粒体丙二醛（MDA）生成的抑制率为38％，而相同浓度下，阳性对照药Vit E的抑制率为35％，表明化合物7具有显著的抗氧化作用。

The stems and branches of Hypericum petiolulatum were extracted by alcohol and liquid-liquid extraction. Seven furo- furanlignans were isolated from the ethyl acetate fraction of ethanol extract of H. petiolulatum by using silica gelchromatography, Sepha- dex LH-20 chromatography, medium-pressure liquid chromatography and preparative HPLC. Their structures were identified by the spectroscopic methods as pinoresinol（ 1 ） , medioresinol （ 2 ） , 8-acetoxypinoresinol （ 3 ） , epipinoresinol （ 4 ） , （ ＋ ） -syringaresinol （ 5 ） , （ ＋ ）-1-hydroxysyringaresinol（6） and erythro-buddlenolE （7）. All the isolates were firstly found in H. petiolulatum. In the bioassay, compound 7 showed remarkable antioxidative activity inhibiting Fe ＋2-cystine induced rat liver microsomal lipid peroxidation with inhibi- tory rate 38% at a concentration of 1 × 10 ^-6 mol · L^-1 （ positive control Vit E with the inhibitory rate of 35% at the same concentra- tion）.