Fe_3O_4纳米粒子从枣皮中分离纯化花青素

Separation and purification of anthocyanin from jujube peel by Fe_3O_4 nanoparticle

针对枣皮花青素对铁离子较为敏感的现象,利用Fe3O4纳米粒子具有比表面积大,易于磁分离,对小分子可产生吸附效应等特点从枣皮中分离纯化花青素,并对其吸附条件和洗脱效果进行研究。结果表明;Fe2+:Fe3+摩尔比1.2∶2,定性温度30℃,熟化温度75℃,搅拌速度1000r·min-1,p H 9时,用油酸作改性剂,制备的Fe3O4纳米粒子效果较好;温度70℃,p H 11,时间15min下,最适吸附比为0.6g·g-1,UV检测Fe3O4纳米粒子吸附效果良好;温度20℃,p H 3,时间2h,电动搅拌下,丙酮洗脱可使Fe3O4纳米粒子与枣皮花青素完全分离,解吸附效果良好。结论;采用化学共沉淀法制备的Fe3O4纳米粒子对枣皮花青素具有良好的吸附作用,丙酮洗脱既解决了解吸附技术难题,又有效地回收了吸附剂,且工艺简单,易于操作。

The anthocyanin in jujube peel is sensitive to ironion phenomenon, the anthocyanins from jujube peel were separated and purified by Fe304 nanoparticles which have large specific surface area, easy magnetic separation, and effective adsorption of small molecules.Tthe adsorption and eluting of Fe3O4 nanoparticles were studied. The results of best synthesis of Fe3O4 nanoparticles was; the molar ratio of Fe^2＋： Fe^3＋ was 1.2 ： 2; temperature 30℃, reaction temperature 75℃, stirring speed 1000r · min^-1, pH value 9. It is better to preparation Fe304 nanoparticles with modifier for oleic aeid; At 70℃, pH 11, 15rain, the optimum absorption ratio of the nanoparticles was 0.6g· g ^-1, adsorption effect of was detected by UV detection ; Fe3O4 nanoparticles and anthocyanin are completely separated with acetone eluting at 20℃, pH 3 for 2h with electric stirring. The effect of desorption was good. Conclusion ： By chemical co- precipitation method to preparation the Fe3O4, nanoparticles has good adsorption effect on anthocyanin, acetone eluting efficiently recovered the adsorbent, The process is simple and easy to operate.