黄芪多糖的提取及抗氧化作用研究

Study on the extraction and antioxidant effect of astragalus polysaccharides

目的:研究黄芪多糖的提取工艺及抗氧化作用。方法:采用正交实验法对水提醇沉法提取黄芪多糖的提取条件进行优化。采用蒽酮-硫酸法测定黄芪多糖的含量,通过考察黄芪多糖清除羟基自由基的清除率来研究其抗氧化作用。结果:黄芪多糖提取的最佳工艺为:提取时间45min、提取温度100℃、料液比1∶10、提取次数3次,提取率为10.35%;不同浓度的多糖对羟基自由基都具有清除作用,黄芪多糖具有较好的抗氧化作用。

Objective ： The extraction process and antioxidant effect of Astragalus polysaccharides was studied. Methods ： The orthogonal test was used to optimize the extraction conditions of water extract-alcohol precipitation for astragalus polysaccharide. The anthrone-sulfuric acid method was used to determine the extraction rate, and the antioxidant effect was studied by clearance rate of hydroxyl radicals. Results ： The optimum extraction procedure was as follows ： 45 minutes extraction, at 100℃, the ratio of material to liquid 1 ： 10, extraction for three times. Under the above conditions, the extraction rate was 10.35%. Different concentrations ofpolysaccharide all showed the scavenging effect on hydroxyl radicals. Therefore astragalus polysaccharide had antioxidant effect.