微生物法提取马齿苋和银杏叶黄酮及其抗氧化活性研究

Microbiological Method of Extracting the Flavonoids from Purslane and Ginkgo Biloba and Analyses of Its Antioxidant Activity

采用乙醇和微生物的方法对马齿苋(Portulaca oleracea L.)和银杏(Ginkgo biloba L.)叶进行黄酮的提取,并测定粗提液对DPPH自由基的清除作用。结果表明,乙醇提取马齿苋和银杏叶黄酮的提取率分别为2.00%和4.37%,微生物提取马齿苋和银杏叶黄酮的提取率分别为2.07%和4.84%。马齿苋和银杏叶微生物提取液对DPPH自由基的清除作用在相同的稀释倍数下高于乙醇提取液,表明微生物提取植物中黄酮具有一定的可行性和优越性。

Ethanol method and microbiological method were used to extract flavonoids from purslane and Ginkgo biloba. DPPH radical scavenging effect of crude extraction was measured. The results showed that the rate of flavonids extracted from Ginkgo and purslane with ethanol method was 2.00% and 4.37%. That from purslane and Ginkgo biloba with microbial method was 2.07% and 4.84%. Scavenging of DPPH radicals of microbial extract at the same dilution factor was higher than that of ethanol extract, indicating that microbiologial method of extracting the flavonoids from plants had certain feasibility and superiority.