介孔羟基磷灰石纳米粒子的制备及作为蛋白类缓释药物载体的应用

Preparation of mesoporous hydroxyapatite nanoparticles and application as a controllable release carrier of protein

利用气液沉淀和水热法相结合制备介孔羟基磷灰石(HA)纳米粒子,并采用X射线衍射(XRD)、透射电子显微镜(TEM)、比表面分析(BET)、孔径计算(BJH)、傅里叶红外(FT-IR)对其进行表征.研究牛血清白蛋白在羟基磷灰石上的等温吸附,通过对等温吸附曲线的拟合,发现该吸附符合Langmuir模型.同时测定了羟基磷灰石-牛血清白蛋白复合物在不同pH磷酸缓冲液下的体外释放行为,结果表明不同pH下释放率的不同是电荷相斥效应造成的,且体外释放行为符合Korsmeyer-Peppas释放动力学方程,其释放机制符合非Fickian扩散.

Mesoporous hydroxyapatite （HA ） nanoparticles were synthesized via gas-liquid chemical precipitation combining with hydrothermal method .And the structure ,morphology and pore size distribution of HA were observed and measured using the X-ray powder diffraction （XRD ） , transmission electron microscopy （TEM ） , Brunauer-Emmett-Teller （ BET ） , Barret-Joyner-Halenda scheme （ BJH ） and Fourier transform infrared spectrophotometer （FT-IR） .The adsorption isotherms of bovine serum albumin （BSA） on as-prepared HA at various temperatures were obtained and the corresponding equilibrium data fitted better the Langmuir model better .Meanwhile ,the BSA cumulative release rate of the BSA-loaded HA was investigated .The release rate was different at different pH values due to the effect of charge repulsion .Furthermore ,the vitro release was in accordance with the Korsmeyer-Peppas equation and the release mechanism fitted the non-Fickian diffusion .