HPLC法同时测定小儿双解止泻颗粒中葛根素和黄芩苷的含量

Simultaneous Determination of Puerarin and Baicalin in Xiao'er Shuangjie Zhixie Granules by HPLC

目的：建立HPLC法同时测定小儿双解止泻颗粒中葛根素和黄芩苷含量的方法。方法：色谱粒为Agilent Zorbax SB-C18柱（250 mm ×4.6 mm,5μm）；流动相A为0.1%的磷酸溶液,流动相B为甲醇,二元梯度洗脱；检测波长：265 nm；流速：10 ml·min-1；柱温：30℃；进样量20ml。结果：葛根素进样量在0.121~1.942μg范围内（r=0.9995）,黄芩苷进样量在0.230~3.686μg范围内线性关系良好（r=0.9995）；平均加样回收率分别为98.10%和98.20%,RSD分别为1.34%和1.11%（n=6）。结论：该法简便、快速、准确,可用于小儿双解止泻颗粒的质量控制。

Objective：To establish a method for the determination of puerarin and balcalin in Xiao’ er Shuangjie Zhixie granules by HPLC. Methods：An Agilent Zorbax SB-C18 column （250 mm × 4. 6 mm,5 μm） was used. The mobile phase A was 0. 1% phos-phoric acid solution and the mobile phase B was methanol with gradient elution. The detection wavelength was 265 nm,the flow rate was 1. 0 ml·min-1 ,the column temperature was 30℃ and the sample size was 20 μl. Results： The linear range of pueratin was 0. 121-1. 942 μg （r=0. 999 5）and that of balcalin was 0. 230-3. 686 μg（r=0. 999 5）. The average recovery was 98. 10% and 98. 20%with RSD of 1. 34% and 1. 11%（n=6）, respectively. Conclusion：The method is convenient and accurate, which can be used in the de-termination of pueraln and balcalin in Xiao’ er Shuangjie Zhixie granules.