HPLC法测定五层共挤输液用袋中抗氧剂1178的含量

Determination of Antioxygen 1178 in 5-Layer Co-extrusion Bags Used for Infusion by HPLC

目的：建立五层共挤输液用袋中抗氧剂1178的含量测定方法。方法：色谱柱：Uitimat XB-C8（250 mm ×4.6 mm,5μm）,流动相：甲醇-水梯度洗脱,流速：1.0 ml·min-1,检测波长：223 nm,柱温：35℃,进样量20μl。结果：抗氧剂1178在1.64~205.10μg·ml-1范围内线性关系良好（r=0.9999）,平均回收率为92.05%,RSD=1.94%（n=9）。结论：建立的方法结果准确、稳定性好、专属性强,可用于五层共挤输液用袋中抗氧剂1178的含量测定。

Objective：To establish a method for the determination of antioxygen 1178 in 5-layer co-extrusion bags used for infu-sion. Methods：A Uitimat XB-C8 （250 mm × 4. 6 mm,5μm） column was used,the mobile phase was methanol-water with gradient elu-tion, the flow rate was 1. 0 ml·min-1 ,the detection wavelength was 223 nm,the column temperature was 35℃ and the injection vol-ume was 20 μl. Results：The concentration of antioxygen 1178 had the linear relationship within the range of 1. 64-205. 10 μg·ml-1 （r=0. 999 9）,and the average recovery was 92. 05% （RSD=1. 94%, n=9）. Conclusion：The method is accurate,stable and spe-cific,and can be used to determine antioxygen 1178 in 5-layer co-extrusion bags used for infusion.