壳聚糖加首乌、丹参和牛膝对实验性脂肪肝大鼠的保护作用

Protective effect of chitosan plus Polygonum multiflorum, Salvia miltiorrhiza, and Achyronthes bidentata on rats of experimental fatty liver

目的 :观察壳聚糖加中药首乌、丹参和牛膝对实验性脂肪肝大鼠的预防作用。方法 :在小剂量四氯化碳致肝损伤的基础上 ,合并应用高脂饮食复制大鼠脂肪肝模型 ,同时分别给予不同浓度的药物 ,以肝脏的脂质含量为衡量标准 ,并应用计算机彩色图象分析技术测量脂肪变性 (脂变 )面积 ,观察药物预防效果。结果 :四氯化碳损伤肝脏合并高脂饮食可引起大鼠肝脏明显的脂变 ,表现为肝组织三酰甘油 (TG)、总胆固醇 (TC)含量显著增加 ,SOD活性明显降低 ,病理切片检查显示肝细胞肿胀 ,内部充满大小不等的脂滴 ,而药物防治组大鼠与模型组相比肝组织TG和TC值显著降低 ,肝细胞的脂变程度明显小于模型组 ,表现为脂滴减少 ,脂变面积缩小 ,SOD活性逐渐升高并恢复正常 ,其改善程度随用药剂量的增加而增加。结论 :该药物对脂肪肝的形成具有明显的预防作用。

AIM: To investigate the protective effects of chitosan plus Polygonum multiflorum, Salvia miltiorrhiza and Achyronthes bidentata on rats of experimental fatty liver. METHODS: Male Sprague Dawley rats were treated with a low dose of CCl 4 and fed by a high fat diet to induce fatty liver model. At the same time, the treated groups were fed with chitosan, Polygonum multiflorum, Salvia miltiorrhiza , and Achyranthes bidentata at different concentrations. The steatosis degree was determined by the contents of lipids in the rat liver. RESULTS: Combined use of CCl 4 and high fat diet increased significantly the contents of triglyceride(TG), total cholesterol(TC) in liver, and the activity of superoxide dismutase was obviously decreased while the treated groups displayed a less steatosis degree and lower TG, TC compared to model group. Moreover, the depleted activity of superoxide dismutase(SOD) in fatty liver was recovered with the reduce of TG and TC when treated with the drugs. CONCLUSION: The results show that chitosan plus Polygonun multiflorum, Salvia miltiorrhiza, and Achyranthes bidentata have protective effects on liver's steatosis.