大鼠腮腺主导管结扎损伤诱导腺体萎缩的组织学变化

Histological changes in atrophy of the rat parotid gland after duct-ligation

目的:研究大鼠腮腺主导管结扎损伤诱导腺体萎缩的组织学变化规律。方法:建立54只Wistar大鼠右侧腮腺主导管结扎损伤诱导腺体萎缩的动物模型,分别于结扎术后0、1、3、7、14和30天获取各组腮腺组织,阿新蓝—过碘酸雪夫(AB-PAS)特染检测腺小叶内酶原颗粒变化,马松三色(Masson)特染检测腺小叶内纤维结缔组织变化,免疫组织化学法检测腺体内半胱氨酸天冬氨酸蛋白水解酶3(Caspase3)和增殖细胞核抗原(Ki一67)的表达。结果:主导管结扎后,AB-PAS/Masson观察见导管结扎后腺泡细胞萎缩、消失,酶原颗粒减少,腺小叶内纤维结缔组织逐渐增多;免疫组织化学染色示Caspase3在主导管结扎7d组达峰值;Ki一67在主导管结扎3d组达峰值。各实验组间Caspase3及Ki-67表达水平差异均有统计学意义(P<0.05)。结论:腮腺主导管结扎后可促使腺泡细胞萎缩、凋亡,分泌功能丧失,腺体逐渐纤维化,从而可避免腮腺区域切除术后涎瘘等并发症的发生。

Objective：To investigate the histological changes in atrophy of the rat parotid gland after duct-ligation. Methods：The excretory ducts were double-ligated with silk suture unilaterally to induce atrophy of the parotid glands of Wistar rats for either 0,1,3 ,7,14 or 30 days respectively. The gland were harvested and examined with AB-PAS and Masson staining. Caspase3 and Ki-67 immunohistochemistries were detected.Results：After duct ligation, the vast majority of acinar cells and the zymogen granules were vanished identified by AB-PAS staining accompanied by increased collagenous fibers. Caspase3 and Ki-67 positive cells were found occasionally in the sham operation group, but were extensively found during the atrophy of parotid glands. The expressions of Caspase3 and Ki-67 positive cells were statistically different between the two groups（P〈0.05）.Conclusion：During atrophy of the parotid gland, most acinar cells and zymogen granules vanished accompanied by increased collagenous fibers. The salivary fistula could be avoided by ligate the stensen’s duct in surgeries of parotid region.