HPLC法测定复方氨林巴比妥注射液中安替比林的含量

Determination of antipyrine compound Amionprine and Antipyrine injection by HPLC method

目的：建立HPLC法测定复方氨林巴比妥注射液中安替比林含量的方法。方法：采用sinochrom ODS-AP（4.6mm×200mm,5μm）色谱柱;流动相：甲醇-1%醋酸溶液（用三乙胺调节p H值至3.7）（45∶55）;流速：0.8ml/min;检测波长：259nm;柱温：40℃;进样体积：10μl。结果：安替比林浓度在0.4~2μg/ml时,线性关系良好（r=0.9999）;平均加样回收率为98.95%（n=9）。结论：该法简便、快速、专属性强、准确度高,适用于复方氨林巴比妥注射液中安替比林的含量测定。

Objective： To establish an HPLC method for determination of antipyrine Compound Amionprine and Antipyrine injection.Method： An sinochrom ODS-AP column（4.6mm × 200 mm,5μm） was used with the temperature of 40℃.The mobile phase consisted of methanol： 1% acetic acid solution（adjusted to p H 3.7 with Triethy Lamine）（45 ∶ 55） and the flow rate was0.8ml / min.The detection wavelength was 259 nm,sample amount to 10μl.Results： the linearity was good in the range of 0.4 ~2μg / ml（r = 0.9999）; The average sample recovery rate was 98.8%（n = 9）.Conclusion： This Method is simple,rapid,specific and accurate,and can be used for determination of compound Amionprine and Antipyrine injection.