HPLC测定贵州不同产地八月瓜中齐墩果酸的含量

Determination of different regions of Guizhou in August melon oleanolic acid content in HPLC

目的：建立HPLC测定贵州不同产地八月瓜中齐墩果酸含量的方法。方法：以70%乙醇90℃水浴回流1h,过滤,滤液挥干,加浓盐酸,70%乙醇回流4h。流动相：甲醇-水-冰醋酸-三乙胺（87∶13∶0.04∶0.02）;检测波长210nm;流速为1.0ml/min;柱温为30℃。结果：齐墩果酸分离效果好,在0.0654~2.616μg（r=0.9999）呈良好线性关系。结论：不同产地八月瓜中齐墩果酸含量存在一定差异。

Objective To establish the content of oleanolic acid in different places of Guizhou in August melon in the determination method of HPLC.M ethods Using 7 0 % ethanol,9 0 ℃ water bath reflux for 1 h,the reflux for 1 times,filtering,adding concentrated hydrochloric acid,7 0 % ethanol,refluxing for 4 h,the mobile phase： methanol water acetic acid-three triethy lamine（8 7 ∶ 1 3 ∶ 0.0 4 ∶ 0.0 2）; the detection wavelength of 2 1 0 nm; the flow rate was 1 ml / min; column temperature was 3 0 ℃.Results Oleanolic acid separation effect is good,in the 0.0 6 5 4 ~ 2.6 1 6 μg（r = 0.9 9 9 9） showed a good linearrelationship.Conclusion There were significant differences among different areas in the content of oleanolic acid in August melon.