葡萄汁中抗肿瘤成分反式白藜芦醇含量的测定及其影响因素

Determing the Content of the Anti-cancer Component trans-Resveratrol in Grape Juice and Its Influence Factors

目的:利用高效液相色谱法(HPLC)测定葡萄汁中抗癌成分反式白藜芦醇的含量,并研究其环境影响因素。方法:选取ZORBAX-C18(250mm×4.6mm,5μm)色谱柱;流动相:70%水-30%乙腈;波长:306nm;流速:1ml/min;进样量:20μl,葡萄汁经滤膜过滤后直接进样测定其中的反式白藜芦醇含量;并采用相同方法研究光照、温度及密封状况对其含量的影响。结果:淳果篮浓缩紫葡萄汁中反式白藜芦醇的含量为(1.64±0.07mg/l),汇源百分百的含量为(0.25±0.01mg/l),统一葡萄多中未检测到反式白藜芦醇。另外,阳光照射、升高温度、密封状况差都会导致反式白藜芦醇含量下降。结论:不同品牌的葡萄汁中反式白藜芦醇含量相差较大;低温避光密封条件下可减少葡萄汁中反式白藜芦醇的损失。

Objective To determine the content of trans-resveratrol,an anti-cancer active ingredient in grape juice,and to analyze the environmental influence factors of its content by high-performance liquid chromatography（HPLC）.Methods All samples were determined by ZORBAX-C18column（250 mm × 4.6mm,5μm） at UV 306 nm wavelength,using 70% water-30% acetonitrile as the mobile phase.The determination of grape juice was carried out by direct injection.After the determination,the same chromatographic conditions and methods were used to study the influence of light,temperature and sealed condition on the content of trans-resveratrol.Results The concentrations of trans-resveratrol in Huiyuan grape juice and Chunguolan purple grape juice were（1.64 ± 0.07mg/L） and（0.25 ± 0.01mg/L）,respectively.No trans-resveratrol was found in Tongyi grape juice.The results show that,sunlight,high temperature and poor seal condition decreased the content of trans-resveratrol.Conclusion The content of trans-resveratrol varies in different brands of grape juice.The grape juice should be stored in cool,dark and sealed conditions to maintain the content of trans-resveratrol.