27例HIV抗体检测不确定者结果特征及跟踪随访分析

Characteristics and follow- up analysis of 27 cases with HIV antibody indeterminate- result

目的探讨＂HIV抗体不确定＂结果和跟踪随访的特点,揭示WB确证试验存在的问题,并提出改进措施。方法回顾分析和评价抗体筛查、受检者随访复检及WB试验各种带型对判断HIV抗体不确定的效果。结果 27例HIV抗体不确定者的样本中,其人群分布既有一般人群,也有高危人群;S/CO值从低到高都有分布,其中S/CO值在0~2.9之间较多,占77.78%;另2例S/CO≥6样本WB结果为阴性,HIV初筛阳性S/CO值的高低不能做为确证阳性的判断依据;抗体不确定样本中WB带型以单一P24带型最多,占59.3%,是WB试验的假阳性主要因素。结论引起HIV抗体不确定结果的因素是多样的,除加强实验室质量控制和对不确定结果受检者的追踪随访外,必要时应结合其他检测技术及流行病学调查结果,从而作出准确诊断。

Objective To discusse HIV antibody indeterminate results and follow -up features, reveal the WB validation test problems, and put forward the measures of improvement. Methods Retrospective analysis and evaluation of antibody screening, subjects retested and various type of WB test judge HIV antibody indeterminate results. Results Among 27 ca- ses of HIV antibody indeterminate, the population distribution had both the general and high risk population. S/CO value from high to low was distributed, the S/CO value was between 0 ~ 2.9 more, accounted for 77.78%. The other 2 patients with S/CO I〉 6 samples WB were negative. HIV positive S/CO value cannot be used as a basis for judging the positive con- firmation. Antibody indeterminate samples with type WB with a single P24 belt type was the most, accounting for 59.3%, which was the WB test false positive factors. Conclusion Factors causing HIV antibody indeterminate results were di- verse. Strengthening the quality control of laboratory and following up, the cases,when necessary, should be combined with other detection and epidemiological survey results, so as to make accurate diagnosis.