反相高效液相色谱法测定尿素乳膏的含量

Determination of Urea in Compound Urea Cream by RP-HPLC

目的建立尿素乳膏的反相高效液相色谱测定方法。方法以甲醇为溶剂,超声提取,冰浴,过滤,制备供试品。色谱柱为Kromasil 100-NH2柱（250 mm×4.6 mm,5μm）,流动相为乙腈：水（98∶2）,检测波长190 nm,流速1.0 m L/min,柱温30℃。结果尿素乳膏在0.375～3.75 mg/m L浓度范围内呈良好的线性关系,r=0.999 98;平均回收率为99.6%（n=9,RSD=0.78%）。结论此方法操作简便、快速准确,可用于尿素乳膏的质量控制。

OBJECTIVE To establish an RP-HPLC method for the determination of urea in compound urea cream. METHODS Urea was extract by methanol with ultrasonic extraction,ice bathing,filtered. The chromatographic column was Kromasil 100-NH2（ 250mm×4.6 mm,5 μm）,Acetonitrile-water（ 90 ∶2） was used as solvents. RESULTS There was a good linearity in the range of 0.375-3.75 mg / m L（ r = 0.999 98）. The average recovery was 100.3%,with RSD = 1.13%. CONCLUSION The method was simple,fast and accurate for the quality control of compound urea cream.