腮腺肿瘤及瘤旁腺泡细胞增殖与细胞凋亡的定量研究

A quantitative study on cell proliferation and apoptosis in parotid tumor and its′contiguous acini

目的 探讨细胞增殖与细胞凋亡在腮腺肿瘤及瘤旁腺泡中的作用。方法 取 44例腮腺肿瘤及瘤旁腺泡作流式细胞术 (FCM )研究。以ModFitLT软件进行细胞增殖分析 ,以Sub -G1法检测凋亡率。结果 腮腺恶性肿瘤的增殖指数 (PI)与凋亡率分别为 2 6 .18%和 2 9.0 2 % ,属最高值 ,其次是良性肿瘤瘤旁腺泡分别为 14 .30 %和 13.41% ,恶性肿瘤瘤旁腺泡分别为 13.45 %和 12 .6 5 % ,良性肿瘤为 9.6 2 %和 9.5 6 %。正常腮腺为 8.0 6 %和6 .6 3%。总体上具有显著性差异 (p <0 .0 5 )。结论 细胞增殖与细胞凋亡在腮腺肿瘤中处于高值 ,而且在腮腺肿瘤瘤旁腺泡中亦处于高值 ,提示瘤旁腺泡可能处于“准新生物”状态 ,它们可能成为肿瘤复发和恶变的发源地。

Objective To determine the effection of proliferation and apoptosis in parotid tumor and its′ contiguous acini .Methods 44cases of parotid tumors and their contiguous acini were studied with flow cytometry ,analysis of cell proliferation by ModFit software and cell apoptosis by sub -G 1 way . The PI and apoptosis rate of malignant tumor were 26.18and 29.02 respectively that is highest value.Next to benign tumor,they were 14.30and 13.41separately, 13.45and 12.65 belong to contiguous acini of carcinoma . 9.62and 9.56 to contiguous acini of benign tumor.But they were only 8.06 and 6.63in normal acini respectively.In which they were obvious different (P< 0.05)in total.Results Conclusion Cell proliferation and apoptosis are in higher value not only in parotid tumors but also in its′contiguous acini .That indicate the contiguous acini maybe in the condition of 'paraneoplasm'.which play an important role in recurrent or malignant change and is removed in operation .