摘要
为研究石蒜碱诱导白血病K562细胞凋亡机制,采用体外细胞培养方式,通过CCK-8法描绘细胞生长曲线,测定细胞增殖活性,CCK-8结果显示石蒜碱能够抑制K562细胞生长,且呈现剂量依赖性,其IC50=4.13μmol/L.倒置显微镜进行细胞形态学观察,石蒜碱作用细胞后,细胞形态学发生改变.PI单染法检测细胞凋亡率,数据显示,石蒜碱诱导K562细胞的凋亡率与给药剂量呈正相关.Western-blot法检测P53蛋白表达情况,随着石蒜碱浓度的增加,P53活性增强(P<0.05).结果显示,石蒜碱抑制白血病K562细胞增殖,诱导细胞凋亡,其凋亡诱导作用可能与细胞内P53基因的表达有关.
The mechanism of lycorine inducing leukemia K562 cell apoptosis was studied by discribing cell growth curve and determining cell proliferation activity through the CCK-8 method.The results showed that lycorine can inhibit the growth of K562 cells and present the dose dependent.The IC50 was 4.13 μmol/L.Cell morphological observation was performed by inverted microscope.Morphological changes could be observed when treated with lyco-rine.The cell apoptosis rate was detected by PI single staining data showed that the rate of lycorine-induced apoptosis of K562 cells is positively correlated to the dose.The P53 pro-tein expression was detected by Western blot method.With the increase of lycorine concen-tration, P53 activity increased (P &lt;0.05).All data indicated that lycorine inhibits leuke-mia K562 cell proliferation and induces cell apoptosis.Its apoptosis may be related to the ex-pression of P53 gene in the cell.
出处
《哈尔滨商业大学学报(自然科学版)》
CAS
2015年第2期135-139,169,共6页
Journal of Harbin University of Commerce:Natural Sciences Edition
基金
国家自然科学资助基金项目(81274067)
黑龙江省自然科学资助基金项目(D201138)
教育部科学技术研究重点项目(211045)
教育部博士点基金项目(20112332110003)
哈尔滨市青年科技创新人才专项资金(2013RFQXJ150)