雌激素硫酸化相关酶SULT1E1和STS与子宫平滑肌瘤发病的研究

Study on estrogen sulfation enzymes-SULT1E1 and STS in pathogenesis of uterine leiomyoma

目的:探讨雌激素硫酸化相关酶硫酸基转移酶(SULT1E1)和硫酸酯酶(STS)蛋白在子宫平滑肌瘤中的表达情况及其在发病机制中的作用。方法:采用免疫组化方法检测28对石蜡包埋的子宫平滑肌瘤组织和肌瘤旁组织中SULT1E1和STS蛋白表达。结果:子宫平滑肌瘤组织中SULT1E1蛋白阳性表达率为39.29%,STS阳性表达率为71.43%,肌瘤旁组织中SULT1E1蛋白阳性表达率为50.0%,STS蛋白阳性表达率为32.14%。子宫平滑肌瘤与肌瘤旁组织比较,SULT1E1和STS蛋白表达差异均有统计学意义。结论:子宫平滑肌瘤组织中SULT1E1低表达、STS过量表达,导致组织局部出现高雌激素水平,这可能在子宫平滑肌瘤的发生、发展过程中起重要作用。

Objective： To explore the expressions of estrogen sulfation enzymes-sulfotransferase （SULT1E1） and sulfatase （STS） in uterine leiomyoma and their roles in pathogenesis of uterine leiomyoma. Methods： Immunohistochemical method was used to detect the expressions of SULT1 E1 and STS proteins in 28 pairs of paraffin-embedded uterine leiomyoma specimens and normal uterine tissue specimens adjacent to leiomyoma. Results： The positive expression rates of SULT1E1 and STS proteins in uterine leiomyoma were 39. 29% and 71.43%, respectively; the positive expression rates of SULT1E1 and STS proteins in normal uterine tissue adjacent to leiomyoma were 50. 0% and 32. 14% , respectively. There were statistically significant differences in the positive expression rates of SULTIE1 and STS proteins between uterine leiomyoma and normal uterine tissue adjacent to leiomyoma. Conclusion： Low expression of SULT1E1 and over-ex- pression of STS in uterine leiomyoma can increase estrogen level, which may play important roles in occurrence and development of uterine leiomyoma.