脉冲电场对宫颈癌Hela细胞黏附和侵袭能力影响的实验研究

Experimental study on the effects of pulse electric fields on adhesion and invasion of cervical cancer HeLa cells

目的:探讨脉冲电场对肿瘤细胞黏附侵袭能力的影响及其可能的作用机制。方法:分别将场强为0 V/cm、500 V/cm、1 000 V/cm和1 500 V/cm的脉冲电场作用于宫颈癌Hela细胞,采用基质胶黏附实验观察脉冲电场对Hela细胞黏附能力的影响;采用Transwell小室实验观察脉冲电场对Hela细胞侵袭能力的影响;采用免疫荧光法检测脉冲电场作用后Hela细胞中MMP-2、TIMP-2的表达情况。结果:经脉冲电场作用后,Hela细胞黏附能力下降,侵袭能力受到抑制,MMP-2的表达水平下调,TIMP-2的表达水平上调。结论:脉冲电场能抑制Hela细胞的黏附和侵袭能力,这种抑制作用可能与其下调MMP-2的表达水平和上调TIMP-2的表达水平有关。

Objective： To explore the effects of pulse electric fields on adhesion and invasion of cervical cancer HeLa cells and the possible mechanism. Methods： Pulse electric fields with intensities of 0 V/cm, 500 V/cm, 1 000 V/cm, and 1 500 V/cm were used to treat cervical cancer HeLa cells, stromal adhesion experiment was used to observe the effects of pulse electric fields on adhesion of cervical cancer HeLa ceils; Transwell assay was used to observe the effects of pulse electric fields on invasion of cervical cancer HeLa cells ; immuno- fluorescence method was used to detect the expressions of matrix metalloproteinase-2＇ （ MMP-2 ） and tissue inhibitor of metalloproteinase 2 （TIMP-2） in cervical cancer HeLa ceils after treated by pulse electric fields. Results： After treated by pulse electric fields, adhesion ability of cervical cancer HeLa cells decreased, the invasion ability was inhibited, the expression level of MMP-2 was down-regulated and the expression level of TIMP-2 was up-regulated. Conclusion： Pulse electric fields can inhibit adhesion and invasion of cervical cancer HeLa cells, which may be correlated with down-regulation of MMP-2 and up-regulation of TIMP-2.