基于高通量测序技术的细菌非编码RNA研究方法进展

Progress in Methodology of Bacterial Noncoding RNA Research Based on High-throughput Sequencing

细菌非编码RNA指细菌中不编码蛋白质,而以RNA的分子形式起调控作用的一类核酸分子。高通量测序技术的应用极大地推进了多种细菌中非编码RNA的发现工作,但由于现阶段对细菌非编码RNA特征的认识尚不够深入,该领域测序数据的生物信息学分析还存在许多不足。介绍了应用高通量测序技术研究细菌非编码RNA的两种主要技术——RNA-seq技术和d RNA-seq技术,对现行的筛选非编码RNA的生物信息学分析方法进行综述,并对该领域生物信息学分析策略的改进提出设想。

Bacterial noncoding RNA refers to the nucleic acid molecules that do not encode proteins, but play roles in the regulation in the form of RNA in bacteria. Application of high-throughput sequencing technology has greatly advanced the discovery of non-coding RNA in a variety of bacterial species. However, due to the lack of enough knowledge on the characteristics of non-coding RNA in bacteria, the bioinformatics analysis of the sequencing data are still defective. This article describes two main techniquesthat applied high-throughput sequencing to be used in the researches on bacterial non-coding RNA：RNA-seq and dRNA-seq. The existing bioinformatics methods for screening of non-coding RNA are reviewed, and the recommendations for improving the bioinformatics strategy in this field are proposed.