胸腺肽α1对脓毒症大鼠Th17细胞的作用

Effect of thymosinα1 on Th17 cells in septic rats

目的：观察胸腺肽α1（Tα1）对脓毒症大鼠Th17细胞的影响。方法采用改良的盲肠结扎穿刺（ CLP）法制作大鼠脓毒症模型，观察Tα1对脓毒症大鼠病死率、Th17细胞、IL－17、血浆白蛋白及血浆C－反应蛋白（ CRP）的影响。结果脓毒症大鼠实验组7 d累积病死率较对照组明显升高；Th17细胞和IL－17的变化趋势相一致，二者均较对照组明显升高；血浆白蛋白浓度较对照组明显降低，CRP浓度较对照组明显升高。给予Ta1后，脓毒症大鼠7 d累积病死率明显下降，Th17细胞和IL－17的表达明显降低，血浆白蛋白浓度明显升高，CRP浓度明显下降。结论 Tα1能通过Th17细胞和IL－17改善脓毒症大鼠的免疫功能、炎性反应和蛋白质代谢，降低死亡率。

Objective To investigate the effect of thymosin α1 on Th17 cells in rats with sepsis.Methods We observed the effect of thymosin α1 on mortality, Th17 cells, IL-17, albumin and C-reactive protein （CRP） in reforming cecal ligation and puncture （CLP） induced septic rats. Results The decreased cumulative 7 -day mortality in septic mice was alleviated by Thymosin α1. Concentration of Th17 cells and IL -17 in CLP -induced septic rats was increased significantly compared to control group.Thymosinα1 significantly decreased Th17 cells and IL-17 concentration in CLP-induced septic rats.Plasma albumin level was decreased in septic mice and the level of plasma albumin was elevated by Thymosinα1.Concentration of CRP increased in both sepsis groups, but was less prominently in thymosin α1 treated group.Conclusion Thymosin α1 can improve immunological function, inflammation condition and protein metabolism through Th17 cells and IL-17.