薯莨水溶提取组分的初步分析

Primary analysis on pigment components of Dioscorea Cirrhosa roots

为了研究薯莨色素提取液的成分,利用凝胶过滤柱色谱法对薯莨水溶提取组分初步分离得到2类组分:乳白色物质和深棕色色素物质。应用诊断试剂、紫外光谱、红外光谱对分离所得的乳白色物质和色素物质进行定性,并结合基质辅助激光解吸飞行时间质谱分析发现:纯水提取的薯莨色素主要是缩合单宁四聚体以下的多聚体;乳白色物质主要是多糖类物质,含有果胶和淀粉,不含蛋白质。采用香草醛-盐酸法和苯酚-硫酸法对提取物中的缩合单宁和乳白色物质中多糖进行定量分析发现,二者在薯莨提取物中的平均含量分别为47.7%和10.2%。

Aiming at exploring the components of the pigment extracted from Dioscorea Cirrhosa roots, the gel column chromatography was used to separate the pigment, and two main types of components were obtained： dark brown pigment and milky white substance. Diagnose reagents reactions, UV-Vis spectra, infrared absorption spectra and MALDI-TOF mass spectra of the products were analyzed. The results showed that the dominant ingredient of the pigment was condensed tannins, which were procyanidin polymers below tetramer. The main ingredient of the milky white substance was polysaccharide, which contained pectin and starch rather than protein. The vanillin-hydrochloric acid method and phenolsulfuric acid method were used to quantify the content of tannins and polysaccharide of pigment from Dioscorea Cirrhosa roots, and the results showed that the average contents of the both were 47.7% and 10.2% . respectively.