β-胡萝卜素-15,15’-单加氧酶(BCMO1)基因多态性与血脂异常的相关性研究

THE ASSOCIATION BETWEEN SINGLE NUCLEOTIDE POLYMORPHISMS OF β,β-CAROTENE-15,15'-MONOOXYGENASE AND DYSLIPIDEMIA RISK

目的探讨β-胡萝卜素代谢酶关键酶β-胡萝卜素-15,15’-单加氧酶(BCMO1)基因常见单核苷酸多态性(SNP)位点基因型与血脂代谢异常之间的关系。方法从宁波市某区体检人群中筛选血脂异常患者(601人)和对照人群(623人),采用询问调查获得对象一般情况及饮食习惯、吸烟、饮酒和体育锻炼等信息,体检获得身高、体重、血压和血脂水平,采用连接酶检测法检测BCMO1基因SNP位点rs12934922、rs7501331、rs6564851和rs11646692基因型。结果χ2检验显示rs11646692位点基因型在对照人群和血脂异常人群间的分布差异有统计学意义(P<0.05)。调整年龄、性别、体质指数、血压和膳食信息等因素后,Logistic回归检验结果,相对于CC型,rs11646692位点CG和GG基因型是血脂异常(OR=0.685,95%CI:0.529–0.887;P<0.01)和低HDL-C(OR=0.612,95%CI:0.420–0.890;P=0.01)的保护因素;rs7501331位点CT和TT基因型是高LDL-C的危险因素(以CC型为参照,OR=1.388,95%CI:1.038–1.857;P=0.027)。结论β-胡萝卜素代谢酶BCMO1的基因多态性对机体血脂代谢有影响。

Objective To examine the association between the single nucleotide polymorphisms（SNPs） of β,β-carotene-15,15＇-monooxygenase（BCMO1）, a key enzyme involved in carotenoid metabolism, and the risk of dyslipidemia. Methods 601 subjects diagnosed with dyslipidemia and 623 control subjects were recruited from the communities in Ningbo, Zhejiang Province. General characteristics, smoking, alcohol consumption, physical activity, as well as dietary intake were collected by questionnaires. Height, weight, blood pressure and blood lipid levels were retrieved from clinical data. BCMO1 SNPs were measured by ligase detection reaction methods. Results χ2 test showed significantly different distribution of rs11646692 genotypes between dyslipidemia and control subjects（P〈0.05）. Logistic regression showed that after adjustment with age, gender, body mass index, blood pressure and dietary intake, CG/GG genotype of rs11646692 was associated with lower risk of dyslipidemia（OR=0.685,95% CI：0.529–0.887;P 0.01; compared with CC genotype） and low HDL-C（OR=0.612,95% CI：0.420–0.890;P =0.01; compared with CC genotype）, and CT/TT genotype of rs7501331 was associated with higher risk of high LDL-C（OR=1.388,95% CI：1.038–1.857;P =0.027; compared with CC genotype）. Conclusion The polymorphisms of BCMO1 may be involved in the regulation of lipoprotein metabolism.