呋喃二烯抗脑胶质瘤的研究

Inhibitory effect of furanodiene on glioma

目的:本文从体内外多角度进行呋喃二烯抑制脑胶质瘤作用的研究及机制的初步探讨。方法:采用四甲基偶氮咗盐微量酶反应比色法(MTT法),研究呋喃二烯对体外培养的人脑胶质瘤U251细胞的增殖抑制作用。呋喃二烯脂质微球的体内抗肿瘤活性研究是通过建立小鼠脑胶质瘤G422整体动物模型,选用昆明种雄性小鼠,呋喃二烯脂质微球(80和40 mg·kg-1),用卡莫司汀(BCNU 20 mg·kg-1)作为阳性对照组,腹腔注射给药,qd,共9次,计算胸腺、脾脏指数及抑瘤率。结果:研究发现FDE浓度为20μg·m L-1作用于U251细胞48 h,对U251细胞抑制率为92.1%,形态学实验表明其能有效诱导细胞调亡。当对G422荷瘤小鼠腹腔给药剂量分别为FDE 80 mg·kg-1和FDE 40 mg·kg-1时,抑瘤率为46.19%和25.56%。结论:呋喃二烯对脑胶质瘤细胞具有很强的抑制和杀伤效应。

Objective ： To investigate the inhibitory effect of furanodiene on glioma in vivo and in vitro, and the possible mechanism. Methods： MTT reduction assay was used to determine the effect of furanodiene on prolif- eration of U251 cells, a human brain glioma cell line. The effect of furanodiene lipid microsphere was evaluated in mice bearing glial G422 tumor. Furanodiene lipid microspheres （80 and 40 mg. kg-1 ） were ip injected into the mice, once daily for 9 days, and BCNU （20 mg·kg-1） was used as a positive control. Thymus and spleen indexes as well as inhibition percentage of the tumor were calculated. Results： Furanodiene treatment for 48 h inhibited proliferation of U251 cells with an inhibition rate of 92.1% , and the morphology showed apoptotic changes. In mice bearing glial G422 tumor, furanodiene lipid 46.19% and 25.56% , respectively. Conclusion cells. microspheres at 80 and 40 mg · kg-1 inhibited tumor growth by ： Furanodiene has a potent inhibitory and killing effect on glioma