不同产地及不同采收期钩藤中异钩藤碱的含量分析

Analysis of the isorhynchophylline in Uncaria rhynchophylla from different regions and different harvest time

目的：研究不同产地及不同采收期钩藤中异钩藤碱的含量。方法应用HPLC测定8个不同产地及不同采收期钩藤中异钩藤碱含量，采用Waters Symmetry C18色谱柱（250 mm34.6 mm，5μm），流动相为甲醇-0.01 mol/L醋酸铵缓冲液（pH 8.0）（60∶40），柱温25℃，进样体积20μl，流速1.0 ml/min，检测波长246 nm。结果不同产地及不同采收期的钩藤中异钩藤碱含量不同，其中含量最高的地区为宁明县；含量最高的采收时间为每年9月至次年2月。结论不同产地钩藤药材异钩藤碱的含量不同，每年9月至次年2月采收的钩藤中异钩藤碱含量较高。

Objective To study the content of isorhynchophylline from different regions and different harvest time.Methods The content of isorhynchophylline in Uncaria rhynchophylla from different regions and different harvest time was tested by HPLC. The Waters Symmetry C18 color （4.6 mm＆#215; 250 mm, 5 μm）, mobile phase of methanol - 0.01 mol/L ammonium acetate buffer （pH 8.0） （60：40）, column temperature 25℃, 20 μl sample volume, velocity of 1.0 ml/min, detection wavelength of 246 nm were setted.Results The isorhynchophylline can be detected in different regions and different harvest time, with the highest content was found in Ningming county and from September to February of the next year.Conclusion The content of isorhynchophylline in Uncaria rhynchophylla varied from different regions and harvest times. The best harvest time is during September to February of the next year.