摘要
目的:提取土人参叶多糖,测定其含量。方法:水提醇沉淀法提取多糖,苯酚—硫酸法显色,以葡萄糖标准液绘制标准曲线。结果:回归方程为y=7.4625x-0.0484,相关系数r=0.9999,平均回收率98.7%,RSD=0.49%(n=5)。结论:土人参叶多糖含量为1.3%,方法简单易行,数据可靠,精密度和稳定性好。
Objective： Extraction of Artemisia capillaries polysaccharide, and content determination of polysaccharide from Talinum paniculatum leaves. Methods： Water extracting and alcohol precipitation extraction for content polysaccharides from Artemisia capillaries. Using phenol - sulfuric acid method to make them development color. With glucose as standard substance. Results： The regression equation for y = 7.4625x- 0.0484, the correlation coefficient r = 0. 9999. The average recovery rate is 98.7 %, RSD = 0.49 % （ n = 5 ）. Conclusion： Artemisia polysaccharide Content is 1.3 % . The results show that the proposed method was easy, accurate, stability and precision weU.
出处
《微量元素与健康研究》
CAS
2015年第3期29-30,共2页
Studies of Trace Elements and Health
基金
国家中医药管理局"十二五"中医药重点学科中药化学建设项目(国中医药人教发[2012]32号)
广西重点学科药物化学建设项目(桂教科研[2013]16号)
2014年右江民族医学院大学生创新训练计划立项资助项目(XJCXB201425)
关键词
土人参叶
多糖
含量测定
苯酚-硫酸法
Talinum paniculatum leaves
polysaccharide
content
phenol - sulfuric acid method
