辣木主枝嫩芽无菌培养及植株再生研究

Sterile Culture of Moringa oleifera Buds and Plants Regeneration Test

[目的]确定不同部位嫩芽外植体与植物生长素及天然提取物诱导辣木不定芽的分化能力及不定根生长的最佳条件。[方法]选择优良辣木主枝嫩芽的茎段为外植体,采用组织培养技术对嫩芽中部茎段诱导、增殖、生根等进行研究。[结果]不同部位的嫩芽是影响辣木诱导和增殖最关键的因素,嫩芽诱导效果:中部>下端>上端,辣木诱导最适宜培养基是MS+6-BA0.5mg/L+马铃薯水汁20g/L+蔗糖25g/L,诱导率为83%;增殖分化培养基为3/4MS+6-BA0.5mg/L+KT0.1mg/L+马铃薯水汁20g/L+蔗糖30g/L,增殖系数为3.63;生根培养基为1/2MS+IBA0.5mg/L+NAA0.2mg/L+香蕉水汁20g/L+蔗糖20g/L,生根率达83%,不定根诱导效果:IBA>IAA>NAA。[结论]以主枝嫩芽中部为外植体可建立辣木嫩芽快繁体系,可提高增殖系数和生根率。

[Objective]Accord with the test make sure different parts of the buds explant and auxin induction and natural extracts of adventitious bud differentiation ability,and the best condition of adventitious roots growth.[Method]Selecting M.oleifera bud as explants,using tissue culture to study the problems such as bud induction,proliferation and rooting.[Result]Different parts of the bud is the key factor to affect induction and proliferation,the buds induction results： middle 〉 lower 〉 upper,the optimum culture medium of M.oleifera is MS ＋ 6-BA0.5 mg/L ＋ The potato water juice 20 g/L ＋ sucrose 25 g/L,induction rate is 83%; the optimum culture medium of proliferation differentiation is 3 /4 MS ＋ 6-BA0.5mg/L ＋ KT0.1 mg/L ＋ The potato water juice 20 g/L ＋ sucrose 30 g/L,k-factor is 3.63; the optimum culture medium of rooting is 1 /2MS ＋IBA0.5 mg/L ＋ NAA0.2 mg/L ＋ banana water juice 20 g/L ＋ sucrose 20 g/L,rooting rate is 83%,adventitious roots induction effect： IBA 〉IAA 〉 NAA.[Conclusion] The central of the main branch buds as explants can establish M.oleifera buds micropropagation system,which can improve the multiplication and rooting rate.