基因敲除GCV2的酿酒酵母构建

Construction of Saccharomyces cerevisiae with Gene Knockout GCV2

[目的]构建低甲醇生成的酿酒酵母工程菌用于酿造低甲醇酒。[方法]利用基因敲除技术,敲除酵母代谢甘氨酸生成甲醇途径的甘氨酸裂解酶系的基因GCV2。[结果]成功构建出基因敲除GCV2的工程酵母L5,用L5酿造酒中的相对甲醇含量为131 mg/L,比初始菌株的192 mg/L降低31%。[结论]利用基因敲除GCV2的工程酵母菌可有效减少酒中由甘氨酸代谢生成甲醇的量。

[Objective] In order to brew low methanol wine,the engineering strain of Saccharomyces cerevisiae was constructed to produce low methanol wine.[Method] The technology of gene knockout was used to knockout GCV2 of glycine cleavage enzyme system in Saccharomyces cerevisiae metabolic pathway of glycine to methanol.[Result] It was succeed to construct the engineering strain L5 of knockout GCV2.The methanol is 131 mg/L in wine brewed by L5.It decreased 31% compared with 192 mg/L methanol in wine brewed by original strain.[Conclusion] It is effective to decrease methanol in wine from glycine metabolized by the Saccharomyces cerevisiae of gene knockout GCV2.