硝酸钠浓度对雨生红球藻生长及虾青素积累的影响

Effect of Na NO_3 Concentrations on Growth of Haematococcus pluvialis and Astaxanthin Accumulation

研究了0.15、0.30、0.75、1.50 g/L 4种不同硝酸钠营养浓度条件对雨生红球藻生长状况的影响,同时探究了该试验条件对雨生红球藻中虾青素起始积累期和后期积累量的影响,以寻找既能使雨生红球藻在氮源用尽后细胞密度达到或接近最大,同时又能最快直接进入虾青素积累阶段的硝酸钠营养添加浓度。试验结果表明,硝酸钠浓度为0.15 g/L时雨生红球藻细胞生长状况最好,细胞浓度最高可以达到5.19×105个/ml。此条件下细胞尚未停止生长即已有细胞开始积累虾青素,变红(培养的第23天)。使用有机溶剂萃取法以丙酮提取虾青素,硝酸钠浓度为0.15 g/L时,最终虾青素浓度达到19.136 mg/L,是其他硝酸钠浓度下的虾青素含量最大值。

Effects of four different Na NO3 concentrations,0.15,0.30,0.75,and 1.50 g/L,on growth of Haematococcus pluvialis and accumulation of astaxanthin were studied.The optimal Na NO3 concentration for growth of H.pluvialis and accumulation of astaxanthin,early as well as high content was determined when nitrogen is depleted.The extracting agent of astaxanthin is acetone in the process.The results showed that the optimal Na NO3 concentration was 0.15 g/L with 5.19 × 105 cells/ml the maximal cell density and 19.136 mg/L the maximal astaxanthin content,while the astaxanthin accumulation started at 23 th day from the culture.