苦参碱对兔耳增生性瘢痕治疗作用的组织形态学研究

TO STUDY THE EFFECTIVENESS OF MATRINE ON THE HYPERTROPHIC SCARRING IN RABBIT EARS BY HISTOMORPHOLOGY

目的:研究苦参碱（matrine,Mat）对兔耳增生性瘢痕治疗作用。方法:利用兔耳腹侧面增生性瘢痕模型,采用组织形态学的方法对比研究苦参碱在兔耳增生性瘢痕形成过程中的影响。结果:兔耳腹侧面增生性瘢痕局部注射苦参碱后,按时间段取材;VG和HE染色显示真皮层明显变薄,成纤维细胞数量明显减少,胶原排列趋于一致;12周后,对照组苦参碱组的增生指数、成纤维细胞数密度、胶原密度分别为3.25±0.89/2.15±0.12,4517.5±879.6/3551.2±234.1,39.89±14.12/28.75±10.87。结论:苦参碱能抑制兔耳增生性瘢痕的增殖过程,使瘢痕组织纤维化程度明显减轻。

Objective: To investigate the effect of matrine on hypertrophic scarring of rabbit ears. Methods: With comparison between the controls and the matrine groups which were injected the matrine in the model of hypertrophic scarring in rabbit ears , to study the changes of histomorphology including hypertrophic Index , Numerical density of fibroblasts on area, Area density of collage on area. Results: The staining of VG, HE show that the subepidermal thickness were reduced , the arrange of collagen tended to unifying condition, the amount of the fibroblasts were inhibited significantly. After 12weeks, the hypertrophic Index , numerical density of fibroblasts on area, area density of collage on area via, that were in the control and matrine groups, the controls were 3. 25 ±0. 89/2. 15 ±0. 12, 4517. 5 ± 879. 6/3551. 2 ± 234. 1, 39. 89 ± 14. 12/28. 75 ± 10. 87 respectively. Conclusion: Matrine is able to inhibit proliferative process of hypertrophic scarring to decease excessive collagen deposition and orient the scarring tissue to normal tissue .