库尔勒香梨叶片不定芽再生诱导的研究

Study on Regeneration of Adventitious Bud from Leaves of Pyrus sinkiangensis Yü

以库尔勒香梨幼叶为外植体,研究基本培养基种类、外源激素浓度、AgNO3浓度及接种叶片的放置方向对叶片不定芽再生的影响。结果表明:培养基的种类是影响叶片能否获得再生不定梢成功的关键,NN69培养基是香梨叶片再生不定芽的最佳培养基,诱导不定芽的分化以培养基NN69+TDZ 1.0mg/L+IBA 0.3mg/L为最佳;附加0.5mg/L AgNO3有利于叶片再生;以叶片远轴面接触培养基比近轴面接触培养基更利于不定芽的再生;结合21d暗培养香梨叶片再生频率最高可达到64.3%,再生芽数最高为2.59。

Taking Korlas frant pear（Pyru ssinkiangensis Yü）young expanded leaves extracted as explants,the effect of element composition of basic medium,the concentration of plant growth regulators,AgNO3 concentration and leaf direction on the medium on shoot regeneration were studied.The results showed that the element composition of basic media was the major factor affecting the shoot regeneration.NN69 medium was the optimal basic medium for the adventitious shoot regeneration from leaves of the Pyru ssinkiangensis Yüand the optimum medium was NN69 medium with 1.0mg/L thidiazuron and 0.3mg/L indole-3-butytric acid.AgNO3 enhanced the regeneration efficiency while its concentration was 0.5mg/L.Adventitious buds was easier induced with the leaf abaxial side touching the medium than with the other side.After 21 days dark culture,the maximum regeneration frequency was 64.3%and the maximum bud number per explant was 2.59.