葵花籽壳水溶性膳食纤维的提取工艺优化及抗氧化活性

Study on the extraction of soluble dietary fiber from sunflower seed shells and its antioxidant activity

以葵花籽壳为原料,提取水溶性膳食纤维,研究提取液浓度、料液比、浸提温度及浸提时间对提取率的影响,通过正交实验优化工艺条件,并对其体外抗氧化性进行研究。结果表明,提取最佳工艺条件为料液比1∶30、氢氧化钠的质量分数9.5%、浸提温度40℃、浸提时间30min,葵花籽壳水溶性膳食纤维提取率可达21.32%。葵花籽壳水溶性膳食纤维对1,1-二苯基-2-三硝基苯肼(DPPH)、超氧阴离子自由基(O2-·)和羟自由基(·OH)表现了良好的清除能力,其清除率在样品浓度为1.0mg/m L时分别为86.67%、70.32%和76.33%。

The optimization of extraction technology of soluble dietary fiber（SDF） in sunflower seed shells and its anti-oxidative activities were studied. Results showed that the optimized extraction conditions were as follows： solid-liquid ratio 1：30,extraction solvent 9.5% NaOH,extraction temperature 40;C,extraction time 30min. Under these conditions,the extraction rate of SDF was 21.32%. The SDF showed strong scavenging activities on 1,1 -Diphenyl-2-picrylhydrazyl radical （DPPH）, superoxide radical （O2 - ） and hydroxyl radical （. OH） methods, the scavenging rates for the radicals were 86.67% ,70.32% and76.33% ,respectively,when the concentration was 1.0mg/mL.