广东紫珠止血谱效相关模式的研究

Spectrum-effect Correlation for Blood Coagulation Activity of Callicarpa kwangtungensis Chun

建立广东紫珠止血谱效相关质量评价模式。使用9种工艺分别制备样品供试液,测定其HPLC指纹图谱;用小鼠剪尾尖法和眼球取血法研究其止血作用。将指纹图谱数据与止血作用结果进行关联,建立数学模型。得到22个与止血作用显著相关的色谱峰,其中苯乙醇苷类化合物12个,黄酮类化合物10个。由建立的数学模型计算得到样品止血时间理论值与实测值的偏差率,78%的偏差率小于10%,28%的偏差率小于5%。论证了广东紫珠中具有止血作用的有效成分是以苯乙醇苷类为主、黄酮类为辅的药效物质基础。

A spectrum-effect correlation pattern was established for evaluating blood coagulation activity of Callicarpa kwangtungensis Chun. The samples were prepared in nine ways and analyzed by HPLC to obtain their fingerprints. Blood coagulation was observed through recording the bleeding time after tail tip amputation and the clotting time of blood samples collected after eye extirpation. A feasible mathematical model was built to correlate the HPLC fingerprint data and blood coagulation. Twenty-two correlation peaks were obtained, which consisted of twelve phenylethanoid glycosides and ten flavonoids. The deviations between the theoretical and measured hemostatic time were calculated through the mathematical model. The results showed that the standard deviation rates of 78 % samples were within 10 %, and those of 28 % samples were within 5 %. It demonstrated that the blood coagulation activity of Callicarpa kwangtungensis Chun was contributed mainly by phenylethanoid glycosides and partly by flavonoids.