食品中叶绿素铜钠测定方法的改进分析

Improvement of Determination Method for Sodium Copper Chlorophyll in Food

本方法首次采用了405nm测定食品中叶绿素铜钠的含量,得到405nm波长下的标准曲线更具有稳定性。本方法的标准曲线方程为Y=0.0486x-0.0055,相关系数达到0.9998。与国标方法的最低检出限0.001g/Kg相比,本方法实测检出限为0.0005g/Kg。同时通过加大解析液用量至75m L,提高样品定容体积至100m L,使改进后回收率均有提高,标准偏差均小于0.05%,不但确保了样品测定的重现性和稳定性,还提高了方法的精密度与准确度。

In this method, 405 nm measure wavelength was adopted at first time to get a more stable standard curve. The standard curve equation is y=0.0486 x-0.0055, and the correlation coefficient acquired 0.9998. The detection limit for this method is 0.0005g/Kg which is much lower than 0.001g/Kg for the national standard method. Besides, by increasing the amount of analytical solution to 75 mL, and the sample volume to 100 rnL, the improved recovery rate were increased and the standard deviation is less than 0.05%. This method not only ensured the reproducibility and stability, but also improved the precision and accuracy for detecting sodium copper chlorophyll in food.