甲状腺乳头状癌组织中 miR-21的表达与临床预后的关系

Expression of miR-21 in papillary thyroid carcinoma and correlations thereof with extrathyroidal invasion and prognosis

目的：检测甲状腺乳头状癌（PTC）肿瘤组织miR-21的表达，探讨miR-21在PTC表达对患者预后的影响。方法采用实时荧光定量聚合酶链反应（RT- PCR）检测手术切除的68例PTC肿瘤组织及癌旁正常组织中miR-21表达情况，分析甲状腺癌组织中miR-21的表达与临床病理参数的关系以及对患者预后的影响。结果 PTC患者肿瘤组织中miR-21的表达水平显著高于癌旁正常组织（P 〈0.05），PTC肿瘤组织中miR-21的表达水平与肿瘤腺外侵润有关（P 〈0.05），PTC患者肿瘤组织中 miR-21表达水平高者，其术后的复发转移率高于低表达者（P 〈0.05）。结论 miR-21在PTC患者肿瘤组织中的表达明显升高，其表达水平与肿瘤腺外侵润、术后复发转移有关。

Objective To investigate the expression of miR-21 in papillary thyroid carcinoma （PTC） tissue and to explore the correlations of miR-21 with the invasion and prognosis of PTC. Methods Sixty-eight surgically resected thyroid samples, including tumor tissues and normal tissues adjacent to carcinoma, underwent reverse transcription polymerase chain reaction to detect the expression levels of miR-21 in the different thyroid tissues. The correlations of miR-21 expression with the clinicopathological features and prognosis were analyzed. Results The expression level of miR-21 in the PTC tissue was significantly higher than that in the normal tissue adjacent to carcinoma （P 〈0.05）. The expression level of miR-21 had a correlation with extrathyroidal invasion （P 〈0.05）. The recurrent/metastatic rate of the patients with a higher miR-21 expression in tumor tissue was significantly higher than that of the patients with lower expression level （P 〈0.05）. Conclusion The expression of miR-21 in PTC tissue is significantly higher than that in the normal tissue, and the up-regulated expression of miR-21 is correlated with the extrathyroidal invasion and recurrence/metastasis.