摘要
Objective: To explore the inhibitory effect of He Ne laser irradiation on fibroblast growth of hypertrophic scars in culture. Methods: He Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm 2 and doses of 3 J/cm 2, 30 J/cm 2 , 90 J/cm 2 and 180 J/cm 2 was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done. Results: Repeated irradiation with He Ne laser at dose of 180 J/cm 2 three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P< 0.05 ). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm 2 He Ne laser the cell number in S phase decreased from 51% to 20% and 14% respectively, the cell number in G 0/G 1 phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub G1 phase was 6.7 % and 9.8 % respectively.Conclusions: Repeated irradiation with 180 J/cm 2 He Ne laser can inhibit scar fibroblasts growth in culture. It may be that He Ne laser irradiation causes cell stagnation in G 0/G 1 phase and apoptosis.
Objective: To explore the inhibitory effect of He Ne laser irradiation on fibroblast growth of hypertrophic scars in culture. Methods: He Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm 2 and doses of 3 J/cm 2, 30 J/cm 2 , 90 J/cm 2 and 180 J/cm 2 was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done. Results: Repeated irradiation with He Ne laser at dose of 180 J/cm 2 three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P< 0.05 ). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm 2 He Ne laser the cell number in S phase decreased from 51% to 20% and 14% respectively, the cell number in G 0/G 1 phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub G1 phase was 6.7 % and 9.8 % respectively.Conclusions: Repeated irradiation with 180 J/cm 2 He Ne laser can inhibit scar fibroblasts growth in culture. It may be that He Ne laser irradiation causes cell stagnation in G 0/G 1 phase and apoptosis.
关键词
氦氖激光
成纤维细胞
生长抑制
瘢痕
Laser, He Ne
Cicatrix
Fibroblasts
Growth disorder
Cell cycle
