配伍对乌头汤中乌头碱型生物碱的影响

Effect of Compatibility on Aconitine Type Alkaloids in Wutou Tang

目的:考察在乌头汤中制川乌配伍后对乌头碱型生物碱的影响。方法:采用HPLC-ESI-MS正离子检测模式分析制川乌单煎液及乌头汤中乌头碱型生物碱类成分,对各成分的MS2和MS3谱图进行解析,比较制川乌单煎液与乌头汤中乌头碱型生物碱类成分的区别。结果:制川乌单煎液及乌头汤中检出36个共有成分,制川乌单煎液中有13个成分在乌头汤中未检出,分别为10-羟基焦乌头碱,10-羟基焦乌头原碱,10-羟基焦中乌头原碱,中乌头碱,10-羟基次乌头次碱,10-羟基焦次乌头碱,去氧乌头碱,焦去氧乌头原碱,去氧中乌头次碱,去氧中乌头原碱,3,13-去氧乌头原碱,3,13-去氧焦乌头碱,3,13-去氧焦乌头原碱。乌头汤中10-羟基中乌头原碱在制川乌单煎液中未检出。结论:制川乌配伍后,大量乌头碱型生物碱类成分丢失,成分区别明显。建立的HPLC-ESI-MS可有效分离各种乌头碱型生物碱类成分,提供了各组分的相对分子质量和结构信息。

Objective： To investigate effect of compatibility on alkaloids in Aconiti Radix Cocta from Wutou Tang. Method： HPLC-ESI-MS positive ion mode was adopted to analyze alkaloids in Aconiti Radix Cocta single decoction and Wutou Tang, parsing each component of MS2 and MS3 spectra, thus comparing alkaloids between Aconiti Radix Cocta single decoction and Wutou Tang. Result： Thirty-six common compositions were detected in Aconiti Radix Cocta single decoction and Wutou Tang, 13 ingredients were detected in single decoction, which were not detected in Wutou Tang, such as dehydrared 10-OH-aconitine, dehydrated 10-OH- aconine, mesaconitine and so on. 10-OH-mesaconine in Wutou Tang was not detected in single decoction solution of Aconiti Radix Cocta. Conclusion： A lot of aconitine type alkaloids in Aconiti Radix Cocta are missing after compatibility, ingredients are clearly different. This established HPLC-ESI-MS can effectively separate various aconitine type alkaloids by providing molecular weight and structure information of components.