海拔高度对黔产太子参总多糖含量的影响

Effect of Altitude on Content of Total Polysaccharides in Pseudostellariae Radix from Guizhou Province

目的:通过考察黔产太子参生长区域海拔高度及其总多糖含量,探讨海拔高度对太子参总多糖含量的影响规律,为该药材的质量评价及科学栽培提供参考。方法:采用水提醇沉法提取太子参总多糖,运用UV测定总多糖含量,硫酸-蒽酮法显色,检测波长626 nm。海拔高度采用手持坐标定位仪测定。结果:不同海拔基地太子参总多糖含量差异较大,含量(281 mg·g-1)最高为施秉县牛大场上坝小朝老,含量(23 mg·g-1)最低为清镇市王庄乡罗田村。相关性分析表明太子参总多糖含量与生长区域海拔高度无显著相关关系。结论:黔产太子参总多糖含量普遍较高,海拔高度对总多糖含量无显著性影响,但应避免在海拔过高区域种植。贵州虽为太子参非道地产区,但可作为太子参的主要种植基地。

Objective： To explore relationship between the content of total polysaccharides from Pseudostellariae Radix and altitude by determining height above sea level with the growth area in Guizhou, and provide theory foundation for quality assessment and scientific cultivation of Pseudostellariae Radix. Method： Ultraviolet speetrophotometry was used to dertermine the content of total polysaccharides, total polysaccharides from Pseudostellariae Radix was extracted by water extraction and alcohol precipitation method, sulfuric acid-anthrone was used as sprayed regents and detection wavelength was at 626 nm. Altitudes were determined by global positioning system. Result： The content of total polysaccharides in Pseudostellariae Radix varied significantly in different altitude, it was the highest of 281 mg .g-1 from Shibing county cattle big dam small toward the old, but it was the lowest of 23 mg .g-1 from Luotian, Wangzhuang, Qingzhen city. But the content of total polysaccharides had no relationship with altitude. Conclusion： The content of total polysaccharides in Pseudostellariae Radix from Guizhou is generally high, altitude has no significant effect on the content of total polysaccharides, but high altitude area should be avoided. Although Guizhou is not famous-region for Pseudostellariae Radix, it can be builded as main planting base.