柿树及其11种寄生植物的齐墩果酸、熊果酸含量相关性

Correlation Analysis of Oleanolic Acid and Ursolic Acid Between Diospyros kaki and its Eleven Kinds of Taxilli Herba

目的：考察柿树及其11种寄生植物齐墩果酸、熊果酸含量的关系。方法：采用RP-HPLC对桑寄生药材及其寄主植物齐墩果酸、熊果酸含量进行测定,齐墩果酸、熊果酸采用乙醇超声提取30 min,采用Agilent Eclipse XDB-C18色谱柱,流动相甲醇-乙腈-0.05%乙酸铵水溶液（12∶67∶21）,流速1.0 m L·min-1,检测波长210 nm。结果：齐墩果酸和熊果酸的线性范围分别为2.34~375（r=0.999 9）,5.21~834 mg·L-1（r=0.999 9）,与峰面积呈良好线性关系,平均加样回收率依次为99.57%,99.72%;柿树枝齐墩果酸质量分数为0.382 6~0.541 1 mg·g-1,熊果酸质量分数为0.649 0~1.625 8 mg·g-1,柿树叶齐墩果酸质量分数为1.773 6~4.274 6 mg·g-1,熊果酸质量分数为6.023 7~9.398 0 mg·g-1;瘤果槲寄生枝齐墩果酸质量分数为1.221 4 mg·g-1,熊果酸质量分数为0,瘤果槲寄生叶齐墩果酸质量分数为10.815 8 mg·g-1,熊果酸质量分数为0;棱枝槲寄生齐墩果酸质量分数为7.158 9 mg·g-1,熊果酸质量分数为0;余下9种寄生齐墩果酸和熊果酸质量分数均为0。结论：柿树枝叶均含齐墩果酸、熊果酸。鞘花、三色鞘花、离瓣寄生、油茶离瓣寄生、五蕊寄生、红花寄生、小红花寄生（变种）、广寄生和大苞寄生9种寄生不含齐墩果酸、熊果酸。瘤果槲寄生和棱枝槲寄生含齐墩果酸,不含熊果酸。

Objective： To analyze the component correlation of oleanolic acid and ursolic acid between Diospyros kaki and its eleven kinds of Taxilli Herba. Method： Oleanolic acid and ursolic acid were extracted with ethanol by ultrasound for 30 minutes,and determined by RP-HPLC. Agilent Eclipse XDB C18column（4. 6 mm ×250 mm,5 μm） was used for the separation,with a flow rate of 1. 0 m L·min- 1and column temperature of 25 ℃.The mobile phase were methanol-acetonitrile-0. 05% solution of ammonium acetate（12 ∶ 67 ∶ 21） and detection wavelength was 210 nm. Result： There were good linearity in the range of 2. 34-375 mg·L- 1（r = 0. 999 9） and5. 21-834 mg·L- 1（r = 0. 999 9） for oleanolic acid and ursolic acid,respectively. The average recovery rates were99. 57% and 99. 72% for oleanolic acid and ursolic acid,respectively. In D. kaki,the content of oleanolic acid was 0. 382 6-0. 541 1 mg ·g- 1（branches） and 1. 773 6-4. 274 6 mg · g- 1（leaves）,and ursolic acid was0. 649 0-1. 625 8 mg·g- 1（branches） and 6. 023 7-9. 398 0 mg·g- 1（leaves）. Ursolic acid was not detected in the eleven kinds of Taxilli Herba,and oleanolic acid was found only in Viscum ovalifolium and V. diospyrosicolum.The contents of oleanolic acid were 1. 221 4 mg·g- 1（in branches of V. ovalifolium）,10. 815 8 mg·g- 1（in leaves of V. ovalifolium）,and 7. 158 4 mg ·g- 1（in V. diospyrosicolum）,respectively. Conclusion： The oleanolic acid and ursolic acid were accumulated in the branches and leaves of D. kaki. Oleanolic acid was accumulated in V. ovalifolium and V. diospyrosicolum,but ursolic acid was not accumulated. The oleanolic acid and ursolic acid were not accumulated in Macrosolen cochinchinensis,M. tricolor,Helixanthera parasitica,H.Sampsoni,D. pentandra,S. parasitica,S. parasitica var. graciliflora,Taxillus chinensis and T. maclurei.