摘要
目的：探讨山茱萸药材鲜品与干品总DNA的提取方法,比较鲜、干品总DNA质量,为以后的研究奠定基础。方法：采用改良CTAB法A,B和经典SDS法,提取山茱萸药材鲜品和干品总DNA,对其进行DNA含量测定和电泳检测。结果：改良CTAB法A提取山茱萸药材鲜品与干品总DNA的OD260/OD280比值在1.7~1.9之间,电泳条带清晰,无拖尾,对山茱萸药材鲜品与干品DNA进行综合比较,结果显示鲜品获得的DNA质量高于干品。结论：改良的CTAB法A是分离高质量完整DNA的方法简便、快速,该方法得到的DNA适合下一步分子生物学研究。
Objective： To explore the best total genomic DNA extraction method from the dried and fresh Corni Fructus and to compare the DNA quality as a foundation for future research. Method： The new modified CTAB method, SDS method were used to get DNA, the content of nucleic acid and agarose gel electrophoresis were tested. Result： The OD260/ OD280 ratios of DNA extracted by using the new modified CTAB method A were between 1. 7-1. 9 and the agarose gel electrophoresis results showed that the bands of the DNA were both tight and distinct. Compared with the DNA samples from dried herbs,results showed that DNA quality from fresh herbs was better. Conclusion： New modified CTAB is a simple and fast method for extract the DNA in high quantity and quality from herbs,the obtained DNA by this method is suitable for further studies of molecular biology.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第10期70-72,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技支撑计划项目(2006BAI09B05-8)
河南省教育厅科学技术研究重点项目(12A360003)
河南中医学院苗圃工程(MP2012-05)
关键词
山茱萸
鲜品
干品
DNA提取方法
Corni Fructus
fresh herbs
dried herbs
DNA extraction
