HPLC测定不同产地泽兰药材中齐墩果酸和熊果酸含量

Determination of Oleanolic Acid and Ursolic Acid in Lycopi Herba from Different Regions by HPLC

目的：建立泽兰药材中齐墩果酸和熊果酸的含量测定方法,并对收集自不同产地（海拔15~2 750 m）的泽兰药材进行含量测定,寻找海拔高度与药材质量的关系。方法：采用Agilent TC C18色谱柱（4.6 mm×250 mm,5μm）,流动相甲醇-乙腈-0.2%冰乙酸（78∶10∶12）,流速0.8 m L·min-1,在210 nm处检测,柱温40℃。结果：齐墩果酸和熊果酸的的线性范围分别为0.164~2.46,0.325 6~4.884μg,回归方程分别为Y=5.025×105X＋2.106×103（r=0.999 8）和Y=4.655×105X＋1.013×104（r=0.999 8）,平均加样回收率分别为99.03%（RSD 1.7%）和98.83%（RSD 1.4%）。海拔高度与药材质量呈现一定的正相关性,即海拔越高,泽兰药材中齐墩果酸和熊果酸含量也越高。结论：所建立的含量测定方法简便快速、准确可靠,可用于评价泽兰药材的质量。海拔高度与药材质量的关系可指导泽兰药材种植区域的优选。

Objective： To set up an content determination method for oleanolic acid and ursolic acid in Lycopi Herba by HPLC,and to find the relationship between altitude and medicinal material content. Method：Agilent TC C18column（4. 6 mm × 250 mm,5 μm） was selected,the mobile phase was methanol-acetonitrile-0. 2% glacial acetic acid solution（78 ∶ 10 ∶ 12）,the flow rate was 0. 8 m L·min- 1. The column temperature performed at 40 ℃,and the target component was detected at 210 nm. Result： Oleanolic acid showed a linear ranges at 0. 164-2. 46 μg（r = 0. 999 8） and ursolic acid was 0. 325 6-4. 884 μg（r = 0. 999 8）. The average recovery of the added sample were 99. 03%（RSD 1. 7%） and 98. 83%（RSD 1. 4%）,respectively. Altitude and herbal content showed a certain degree of positive correlation. Conclusion： The method is simple and rapid,accurate and reliable,therefore available for the elevation of Lycopi Herba. Relationship between altitude and quality can be a preferably guidance for planting area.