丹红化瘀口服液抗血栓形成作用

Antithrombotic Effect of Danhong Huayu Koufuye

目的:从抗血栓形成作用初步探讨丹红化瘀口服液(Danhong Huayu Koufuye,DHK)抗糖尿病视网膜病变和糖尿病肾病的作用机制。方法:采用毛细管法检测新西兰兔凝血时间,断尾法检测昆明种小鼠出血时间,同时考察DHK对大鼠下腔静脉结扎诱导的静脉血栓、动-静脉旁路血栓和Fe Cl3诱导颈动脉血栓3种血栓形成的作用。凝血时间、出血时间、下腔静脉血栓和动静脉旁路血栓实验动物分为4组:溶剂组,DHK低剂量组(各实验剂量分别为0.60,2.25,1.60,1.60 g·kg-1·d-1),DHK高剂量组(各实验剂量分别为1.20,4.50,3.20,3.20 g·kg-1·d-1),阿司匹林组(各实验剂量分别为2.88,11.26,7.81,7.81 mg·kg-1·d-1);Fe Cl3诱导颈动脉血栓实验分为5组:假手术组,模型组,DHK低、高剂量组(1.60,3.20 g·kg-1·d-1),氯吡格雷组(30 mg·kg-1·d-1)。凝血时间实验采用单次及连续ig给药5 d后进行指标检测,其余实验均连续ig给药5 d后进行指标检测。结果:与溶剂组比较,DHK高剂量组显著延长新西兰兔凝血时间(P<0.05)和小鼠出血时间(P<0.05)。与模型组比较,DHK高、低剂量组抑制大鼠下腔静脉血栓、动-静脉旁路血栓和Fe Cl3诱导的大鼠动脉血栓的形成(P<0.05)。结论:DHK有明显的活血化瘀和抗血栓形成作用。

Objective： The aim of the study was to investigate effects and mechanism of Danhong Huayu Koufuye （DHK） on diabetic retinopathy and diabetic nephropathy based on the aspect of thrombus formation. Method： Effects of DHK on the clotting time and bleeding time were observed by capillary glass tube method and transecting the tip of the tail, respectively. Antithrombotic effects of DHK were examined with the ligation model of inferior vena cava, carotid artery-carotid vein bypass and ferric chloride-induced arterial thrombosis in rats. The animals in the study of the clotting time, bleeding time, the ligation model of inferior vena cava and carotid artery- carotid vein bypass model were randomly divided into 4 groups： model group, low dosage of DHK （0.60, 2.25, 1.60, 1.60 g.kg-1.d-1）, high dosage of DHK （1.20, 4.50, 3.20, 3.20 g.kg-1.d -1）, aspirin group （2. 88, 11.26, 7.81 , 7.81 mg .kg-1.d-1 ） , respectively. Rats in ferric chloride-induced arterial thrombosis experiments were divided into 5 groups： sham group, model group, low and high dosage of DHK （1.60, 3.20 g .kg -1. d -1 ） , and clopidogrel group （30 mg .kg-1.d-1 ）. Clotting time was assayed after single and multiple administration for 5 days. Bleeding time and the thrombus weight were detected after administered for 5 days. Result： Compared with vehicle control, DHK significantly prolonged the clotting time in New Zealand rabbits （P 〈 0.05） and bleeding time in mice （P 〈 0. 05）. High dosage of DHK significantly inhibited thrombus formation. Inhibition rate of thrombus dry weight in high dosage DHK group was 73.8% in ligation model of inferior vena cava, and 45.1% in carotidartery-the carotid vein bypass model. Thrombus percentage of high dosage DHK group was （52. 1 ± 17.7） % （P 〈0. 05; vs model group） in ferric chloride-induced arterial thrombosis in rats. Conclusion： DHK has activities of promoting blood circulation to remove blood stasis and prevent thrombus formation.