杨桃根提取物对糖尿病小鼠的降糖作用

Hypoglycemic Effects of Averrhoa carambola Root Extract in Diabetic Mice

目的:研究杨桃根提取物(YTG)对链脲佐菌素(STZ)致糖尿病小鼠的降糖作用。方法:SPF级KM雄性小鼠60只,采用尾静脉注射STZ建立糖尿病小鼠模型,将成模小鼠随机分成模型组,二甲双胍组(0.25 g·kg-1·d-1),YTG高、中、低剂量组(1.2,0.6,0.3 g·kg-1·d-1),另设正常组,每组10只,ig给药4周,在第2,4周末测定空腹血糖(FBG),实验末期进行糖耐量试验(OGTT),并测定肝组织中肝糖原含量,己糖激酶(HK),丙酮酸激酶(PK),超氧化物歧化酶(SOD),丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)的水平。结果:与正常组比较,模型组给药FBG明显升高,血糖曲线下面积明显升高,肝糖原,HK,PK水平明显降低,肝组织中MDA含量明显升高,SOD,GSH-Px水平明显降低,均具有明显统计学差异(P<0.01);与模型组比较,各YTG给药组,FBG明显下降,血糖曲线下面积明显降低,肝糖原含量增加,HK,PK活性增加,肝组织中SOD和GSHPx活性明显升高,MDA含量降低,均具有统计学差异(P<0.05,P<0.01)。结论:杨桃根提取物能降低糖尿病小鼠的血糖,提高糖耐量,其机制可能与增强HK,PK的活性,提高肝脏的抗氧化能力有关。

Objective： To investigate the hypoglycemic effects of Averrhoa carambola root extract（YTG） in diabetic mice induced by streptozotocin（STZ）. Method： Diabetic mice were induced by intravenous injection of STZ. Mice were divided into the normal group,the model group,the metformin group,the high-,medium-and low-dose YTG groups（1. 2,0. 6,0. 3 g·kg- 1·d- 1） of 10 mice each. Drugs were intragastrically administered to mice for 4 weeks. Fasting blood glucose（FBG） was determined at the 2nd and 4th week. After 4weeks,oral glucose tolerance test（OGTT）,liver glycogen,activities of hexokinase（HK）,pyruvate kinase（PK）,superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） and malonyldialdehyde（MDA） were measured. Result： Compared with normal group,FBG,the area under curve of blood glucose,and MDA content increased significantly,the liver glycogen content and the activity of HK,PK,SOD,and GSH-Px decreased significantly in the model group（P〈0. 01）. Compared with the model group,FBG,the area under curve of blood glucose,and MDA content decreased significantly,while the liver glycogen content and the activity of HK,PK,SOD,and GSH-Px increased significantly in YTG group（P〈0. 05,P〈0. 01）. Conclusion： YTG could reduce the blood glucose level of diabetic mice,improve the glucose tolerance. Its mechanism may be related to enhancing the activities of HK and PK and improving the liver antioxidant ability.