豆茶决明对高脂诱导C57BL/6J小鼠肥胖和胰岛素抵抗的影响

Effect of Cassia nomame on Obesity and Insulin Resistance in High-fat Diet-induced C57BL /6J Mice

目的:探讨豆茶决明对高脂诱导肥胖和胰岛素抵抗的干预作用。方法:雄性C57BL/6J小鼠90只分为6组,即正常组,模型组,豆茶决明低、中、高剂量组(0.1,0.3,0.9 g·kg-1),阳性药组(罗格列酮,0.75 mg·kg-1),每组15只,ig给药。每周称量体重,8周末,禁食不禁水12 h,测量空腹血糖(FBG),处死后测量体长,称量腹部、睾丸及双肾部位脂肪湿重,取腹部脂肪组织制备石蜡切片进行HE染色,测定空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(IRI),检测血清总胆固醇(TCH),甘油三酯(TG)以及白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α)水平。结果:与正常组比较,模型组小鼠体重明显升高,FBG,FINS,血清TCH,TG,IL-6和TNF-α水平明显升高,IRI明显升高,均具有明显统计学差异(P<0.01);与模型组比较,豆茶决明低、中、高剂量组小鼠体重明显下降,FBG,FINS,血清TCH,TG,IL-6和TNF-α水平明显降低,IRI明显减小,均具有明显统计学差异(P<0.05,P<0.01)。病理组织检测显示,各给药组均能明显降低模型组脂肪含量及细胞体积。结论:豆茶决明具有抑制肥胖和胰岛素抵抗的作用。

Objective： To investigate the effect of Cassia nomame on obesity and insulin resistance in high-fat diet-induced C57 BL /6J Mice. Method： The experimental animals were randomly divided into six groups：the normal group,the model group,the low-,middle- and high-dose Cassia nomame groups（0. 1,0. 3,0. 9 g·kg- 1）,and the rosiglitazone group（0. 75 mg · kg- 1） of 15 rats each. All mice received intragastric administration of the corresponding medicines,the body weight of mice was measured every week. After 12-hour fast but water-drinking,the fasting plasma glucose（FBG） of mice was tested at the end of the eighth week. After all experimental animals were executed,body length was measured,the adipose tissue around stomach,testis and kidney were weighed,and the stomach adipose tissue was stained by HE staining. Fasting insulin（FINS） was measured and the insulin resistance index（IRI） was calculated. The levels of total cholesterol（TCH）,triglyceride（TG）,interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） in serum were measured. Result： Compared with normal diet group,the body weight,the levels of FBG,FINS,TCH,TG,IL-6 and TNF-α increased in the model group（P〈0. 01）. Compared with the model group,the body weight,and the levels of FBG,FINS,TCH,TG,IL-6 and TNF-α decreased in the C. nomame group（P〈0. 05,P〈0. 01）. HE staining showed that the fat content and cell volume decreased in the C. nomame group. Conclusion： Cassia nomame could effectively inhibit obesity and insulin resistance in high-fat diet-induced C57 BL /6J mice.